Vernalization of spinach. (I)

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Isolation of photosystem I particles from spinach.

A method to prepare photosystem I (PSI) particles is described. Spinach leaves are used to prepare broken chloroplasts that are then solubilized by using a detergent (Triton X-100). Solubilized chloroplasts are then applied on an ion-exchange column. Eluted by a linear concentration gradient of NaCl, fractions enriched in PSI particles are collected and applied on a small hydroxyapatite column....

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ژورنال

عنوان ژورنال: Engei Gakkai zasshi

سال: 1956

ISSN: 1880-358X,0013-7626

DOI: 10.2503/jjshs.25.167